Most significantly, the downregulation of mutant

Ras abolished the colony growth in vitro and tumor formation in vivo, fully demonstrating that the transfected cancer cells had lost their anchorage dependent growth and tumorigenicity. Another study involving the abolition of activated K Ras in two pancreatic cancer cell lines PANC 1 and MIAPACA 2, via mutant specific siRNA, confirmed similar cellular changes consistent with a reduced malignant phenotype. Both cell lines had a decrease in cell proliferation, reduced cell migration,cheap jerseys vascular endothelial growth factor (VEGF) reduction and colony formation after muted K Ras knockdown, definitely indicating siRNA inhibits pancreatic cancer cells growth in vitro.

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